Collins, H. E., Pat, B. M., Litovsky, S., Young, M., & Chatham, J. (2015). Abstract 19844: Stromal Interaction Molecule 1 is Essential for the Maintenance of Cardiac Metabolism.
Collins, Helen E, Betty M Pat, S. Litovsky, M. Young, and J. Chatham. “Abstract 19844: Stromal Interaction Molecule 1 Is Essential for the Maintenance of Cardiac Metabolism” (2015).
Collins, Helen E., et al. Abstract 19844: Stromal Interaction Molecule 1 Is Essential for the Maintenance of Cardiac Metabolism. 2015.
The ER/SR Ca2+ sensor, Stromal Interaction molecule 1 (STIM1), a key regulator of store-operated calcium entry is expressed in cardiomyocytes and has been implicated in regulating hypertrophic signaling. We have recently shown that mice with a cardiomyocyte-restricted deletion of STIM1 develop dilated cardiomyopathy. Given the importance of Ca2+ in regulating cardiac metabolism we examined whether a lack of STIM1 alters cardiac metabolism. We found that hearts isolated from 20 week crSTIM1-KO mice exhibit significant changes in cardiac metabolism, observed by a significant reduction in both glucose oxidation (0.75 ± 0.06 v 0.46 ± 0.04 μmol/min/g dry wt, p < 0.01) and net lactate release (15.38 ± 1.29 v 10.23 ± 0.87 μmol/min/g dry wt, p <0.02) in an isolated perfused working heart model in comparison to control mice. These changes occurred independent of any changes in contractile function or oleate oxidation (0.22 ± 0.03 v 0.26 ± 0.02 μmol/min/g dry wt, ns). Consistent with a reduction in glucose oxidatio...